Yes you can but if you have a lot of files, a flash drive won't back it all up. If you only need to back up 32GB-64GB for example, you could back it up on the flash drive. However, anything bigger isn't sensible for flash drives; you're better off getting an external hard drive. Also, it's not a long-term storage option––it can corrupt easily or be lost.
Most flash drives ship preformatted with the FAT32, or exFAT file systems. The ubiquity of the FAT32 file system allows the drive to be accessed on virtually any host device with USB support. Also, standard FAT maintenance utilities (e.g., ScanDisk) can be used to repair or retrieve corrupted data. However, because a flash drive appears as a USB-connected hard drive to the host system, the drive can be reformatted to any file system supported by the host operating system.

USB flash drives are only limited by the cost of the storage space and the physical constraints of the drive. Some of the cheaper flash drives can store less than a gigabyte, but modern high-end flash drives can store as much as 1TB of data, about equivalent to a typical hard drive. A standard external USB drive can store or backup around 64GB, 128GB, 256GB, or 512GB of data. As storage space increases, USB flash drives have become proficient at storing massive files such as ultra high-definition videos that display at 4K or even 8K resolution.
Flash drives may present a significant security challenge for some organizations. Their small size and ease of use allows unsupervised visitors or employees to store and smuggle out confidential data with little chance of detection. Both corporate and public computers are vulnerable to attackers connecting a flash drive to a free USB port and using malicious software such as keyboard loggers or packet sniffers.
Artists have sold or given away USB flash drives, with the first instance believed to be in 2004 when the German punk band Wizo released the Stick EP, only as a USB drive. In addition to five high-bitrate MP3s, it also included a video, pictures, lyrics, and guitar tablature.[58] Subsequently, artists including Nine Inch Nails and Kylie Minogue[59] have released music and promotional material on USB flash drives. The first USB album to be released in the UK was Kiss Does... Rave, a compilation album released by the Kiss Network in April 2007.[60]

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As highly portable media, USB flash drives are easily lost or stolen. All USB flash drives can have their contents encrypted using third-party disk encryption software, which can often be run directly from the USB drive without installation (for example, FreeOTFE), although some, such as BitLocker, require the user to have administrative rights on every computer it is run on.
Digital audio files can be transported from one computer to another like any other file, and played on a compatible media player (with caveats for DRM-locked files). In addition, many home Hi-Fi and car stereo head units are now equipped with a USB port. This allows a USB flash drive containing media files in a variety of formats to be played directly on devices which support the format. Some LCD monitors for consumer HDTV viewing have a dedicated USB port through which music and video files can also be played without use of a personal computer.

In the arcade game In the Groove and more commonly In The Groove 2, flash drives are used to transfer high scores, screenshots, dance edits, and combos throughout sessions. As of software revision 21 (R21), players can also store custom songs and play them on any machine on which this feature is enabled. While use of flash drives is common, the drive must be Linux compatible.

Buying your flash drives in bulk may be a way to save in the long run depending on how many individual drives you need. Some brands or distributors may offer you discounts in an effort to move some of their inventories. eBay allows you to sort items by price or input your own custom price range. Using this feature may help you find the deal that works for you. Some standard price ranges you might find by default are the following:
Universal Serial Bus (USB) is a standard that defines both communication protocols and physical connections for data transfer. Using thumb drives based on this standard ensures they work with a broad range of devices, making it simple to show digital pictures on a USB-enabled television, transfer files between Apple and Microsoft computers or use your saved game on a friend's gaming console. These devices also offer plug-and-play compatibility with most operating systems and require no additional software to access the storage they provide.
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