Digital audio files can be transported from one computer to another like any other file, and played on a compatible media player (with caveats for DRM-locked files). In addition, many home Hi-Fi and car stereo head units are now equipped with a USB port. This allows a USB flash drive containing media files in a variety of formats to be played directly on devices which support the format. Some LCD monitors for consumer HDTV viewing have a dedicated USB port through which music and video files can also be played without use of a personal computer.
A recent development for the use of a USB Flash Drive as an application carrier is to carry the Computer Online Forensic Evidence Extractor (COFEE) application developed by Microsoft. COFEE is a set of applications designed to search for and extract digital evidence on computers confiscated from suspects.[48] Forensic software is required not to alter, in any way, the information stored on the computer being examined. Other forensic suites run from CD-ROM or DVD-ROM, but cannot store data on the media they are run from (although they can write to other attached devices, such as external drives or memory sticks).

Flash drives implement the USB mass storage device class so that most modern operating systems can read and write to them without installing device drivers. The flash drives present a simple block-structured logical unit to the host operating system, hiding the individual complex implementation details of the various underlying flash memory devices. The operating system can use any file system or block addressing scheme. Some computers can boot up from flash drives.
Like all flash memory devices, flash drives can sustain only a limited number of write and erase cycles before the drive fails.[71][unreliable source?][72] This should be a consideration when using a flash drive to run application software or an operating system. To address this, as well as space limitations, some developers have produced special versions of operating systems (such as Linux in Live USB)[73] or commonplace applications (such as Mozilla Firefox) designed to run from flash drives. These are typically optimized for size and configured to place temporary or intermediate files in the computer's main RAM rather than store them temporarily on the flash drive.

Some manufacturers deploy physical authentication tokens in the form of a flash drive. These are used to control access to a sensitive system by containing encryption keys or, more commonly, communicating with security software on the target machine. The system is designed so the target machine will not operate except when the flash drive device is plugged into it. Some of these "PC lock" devices also function as normal flash drives when plugged into other machines.
USB 2.0 flash drives have a transfer rate of up to 480Mbps, while USB 3.0 flash drives allow for transfer rates 10 times faster — up to 4.8Gbps. However, you can only take advantage of this higher speed if your device has a USB 3.0 port. Although USB 3.0 flash drives and other devices are backwards compatible with USB 2.0 ports, they will only operate at a USB 2.0 rate.
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